Having graduated from the Choral Conducting Department of the Dmitry Bortniansky Music School in Sumy in 1996, Olena Yatskulynets obtained qualifications as a music teacher. In 1996–2001, she studied at the Ukrainian National Tchaikovsky Academy of Music in Kyiv, where she received diplomas as a Choir Conductor and a Teacher of Choral Disciplines. After completing her studies, she undertook a four-year assistantship at her alma mater.
She was the director of the Vesnyanka Choir (1998–2007), the D. Kabalevsky Music School Choir (2005–11), and the Pokrov Chamber Choir (2002–17). She also served as Music Director of the Kyiv Academic Puppet Theatre (2011–22) and Artistic Director of the Moravski Chamber Choir (2016–22).